Joshua Gross

mongoose-subpopulate. A monkey-patch of the populate Mongoose library for using MongoDB in Node.js apps. Subpopulate allows you to nest populate calls.

objc-ios-benchmarks. Objective-C iOS benchmarks for better understanding of Objective-C and the Objective-C runtime on iOS.

md2anki. Convert markdown files to anki card deck (apkg) files.

Vanity. Vanity page & microblogging based on Node.js

ShareJS. Collaborative editing in any app

jailkeeper. Monitor spawned child processes and make sure they don't read or write to files they shouldn't have access to. Jailkeeper is for managing cloud-based services that need to run potentially unsafe shell scripts.

pysword. A native Python implementation of the SWORD Project Bible Reader

ilc. Untyped incremental lambda calculus

sort-movies.py. Python script to sort, organize, and search through your movies

PrettyDatePicker. Nicer date picker for iOS with today, yesterday, day-of-week selection

html-php-javascript-livelessons. Source code for the HTML, PHP, and JavaScript LiveLessons series, to be published by Pearson/Addison-Wesley - coming soon!

iphone-libs. Various useful libs, classes, and methods for iPhone development.

react-native. A framework for building native apps with React.

STILC. Simply-typed incremental lambda calculus.

cover_me. An RCov-esque coverage tool for Ruby 1.9

react. A declarative, efficient, and flexible JavaScript library for building user interfaces.

rs-kaleidoscope. Kaleidoscope toy language in Rust, using LLVM JIT

pdf.js. PDF Reader in JavaScript

rrvideo. transform rrweb session into video

rrweb. record and replay the web

posthog-react-rrweb-player. TypeScript

puppeteer. Headless Chrome Node.js API

lit-html. HTML template literals in JavaScript

app-log-dir. Node.js: get system-specific application logging directories. Supports Windows, Linux, Mac.

stayinghomeclub. A list of all the companies WFH or events changed because of covid-19

yprof. Yahoo profiler for Node.js.

R_DataProducts_W2. R Data products coursera course, week 2 assignment.

rs-lambda-calculus. An implementation of the untyped lambda calculus - a parser and evaluator - in Rust

CourseraGettingCleaningDataCourseProj. Coursera "Getting and Cleaning Data" data science course - course project

react-three-fiber. 🇨🇭 A React renderer for Three.js

node-fibers. Fiber/coroutine support for v8 and node.

datasciencecoursera.

PotreeConverter. Create multi res point cloud to use with potree

SDWebImage. Asynchronous image downloader with cache support with an UIImageView category

servo. The Servo Browser Engine

ProgrammingAssignment2. Repository for Programming Assignment 2 for R Programming on Coursera

Job. JavaScript

node-zipfile. Inspect and uncompress zipfile archives in Node.js

Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273. RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

ExData_Plotting1. Plotting Assignment 1 for Exploratory Data Analysis

ServerSwap. ServerSwap is a zero-downtime Node.js server restarter/hot-swapper, for use in production environments to push new releases rapidly without incurring any downtime.

wordlesolver. Python

prepack. Prepack is a partial evaluator for JavaScript. Prepack rewrites a JavaScript bundle, resulting in JavaScript code that executes more efficiently.

R_DataProducts_W3. HTML

RepData_PeerAssessment1. Peer Assessment 1 for Reproducible Research

asyncblock. A simple and powerful abstraction of node-fibers

qmk_firmware. Open-source keyboard firmware for Atmel AVR and Arm USB families

obsidian-full-calendar. Keep events and manage your calendar alongside all your other notes in your Obsidian Vault.

markdown-js. A Markdown parser for javascript

libsbp. Swift Binary Protocol client libraries

vim-settings. my vimrc and plugins

cortex-frame. Minimal PHP framework. Template module is compatible with CodeIgniter.